Alkaline protease production by immobilized cells using B. licheniformis

In recent years there has been potential increase in the use of alkaline protease as industrialcatalysts. many major industrial and commercial applications, such as food and textile industries, andmedical diagnoses, are highly dependent on the protease enzyme. in the cell immobilization technique, thefree movement of microorganisms is restricted in the process, and a continuous system of fermentationcan be used. in the present work, this technique has been used for alkaline protease production usingdifferent carriers, such as chitosan, corn cob and corn tassel. enzyme activity before immobilization (72 h)was 78:3 u=ml. corn cob, with 65% immobilization capacity and the highest enzyme activity, was selectedas the best carrier. after immobilization on the corn cob enzyme, activity was obtained (119:67 u=ml).