Detection of amplified SRY gene by a novel electrochemical biosensor based on gold nanoparticles

This paper describes an electrochemical hybridization biosensor that investigates dnahybridization on the modified glassy carbon electrode (gce). gce was modified by a mixture of aunanoparticles (aunps) and calf thymus dna (ctdna). sh-ssdna was self-assembled on a modifiedelectrode, which is a segment of the sex determining region of the y (sry) gene. this electrode candetect the complementary and non-complementary strand of sry gene, by methylene blue (mb), asan electroactive label, and the differential pulse voltammetry (dpv) technique. the modified gce forthe complementary dna had a linear dynamic range of 0.530.0 ppm. this is the first report of sexdetermination by the electrochemical biosensor of a polymerase chain reaction (pcr) product. also, thisbiosensor showed stability on thermal denaturation for five sequential denaturation processes.