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Neurotoxicity of pre-incubated alpha-synuclein with neutral nanoliposomes on PC12 and SHSY5Y cell lines
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نویسنده
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aliakbari f. ,shabani a.a. ,bardania h. ,eslampanah seyedi h.a. ,mohammad-beigi h. ,tayaranian marvian a. ,nasouti m. ,vafaei a.a. ,shojaosadati s.a. ,saboury a.a. ,christiansen g. ,morshedi d.
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منبع
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scientia iranica - 2017 - دوره : 24 - شماره : 6-F - صفحه:3542 -3553
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چکیده
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Alpha-synuclein (ce-syn) is a protein with high tendency to convert into toxic aggregates that are involved in parkinson's disease (pd). the conversion of ce-syn into toxic aggregates may associate with bio-membranes and the interaction between ce-syn and bio-mimic liposomes can trigger the neurotoxicity. some inhibitors of ce-syn aggregation are challengeable drugs. using nano carriers such as nanoliposomes may overcome such challenges. nevertheless, there are few studies on nanoliposomes effects on neuronal cells and ce-syn. herein, we investigated the neurotoxicity and fibrinogenesis of neutral charged nanoliposomes. thin layer evaporation method and hydration method was employed to formulate the nanoliposomes from dipalmitoylphosphatidylcholine to the size of < 100 nm. fibrillation and cytotoxicity of ce-syn treated with nanoliposome were measured. neither neurotoxicity nor fibrillation was observed when ce-syn treated with different concentrations of nanoliposome. these results well indicate that nanoliposome at the concentrations required for drug delivery not only is able to prevent fibrillation process, but also has no considerable toxic effects on the cells.
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کلیدواژه
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Alpha-synuclein; Amyloid-related diseases; Fibrillation; Nanoliposome; Neurotoxicity; Parkinson's disease
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آدرس
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semnan university of medical sciences, school of medicine, student research committee, department & center for biotechnology research, ایران. national institute of genetic engineering and biotechnology, institute of industrial and environmental biotechnology, bioprocess engineering department, ایران, semnan university of medical sciences, school of medicine, department & center for biotechnology research, ایران, yasuj university of medical sciences, cellular and molecular research center, ایران, national institute of genetic engineering and biotechnology, institute of industrial and environmental biotechnology, bioprocess engineering department, ایران, aarhus university, interdisciplinary nanoscience centre (inano), department of molecular biology and genetics, Denmark, national institute of genetic engineering and biotechnology, institute of industrial and environmental biotechnology, bioprocess engineering department, ایران, national institute of genetic engineering and biotechnology, institute of industrial and environmental biotechnology, bioprocess engineering department, ایران, semnan university of medical sciences, research center, school of medicine, department of physiology, laboratory of learning and memory, ایران, tarbiat modares university, faculty of chemical engineering, biotechnology group, ایران, university of tehran, institute of biochemistry and biophysics (ibb), ایران, aarhus university, department of biomedicine, Denmark, national institute of genetic engineering and biotechnology, institute of industrial and environmental biotechnology, bioprocess engineering department, ایران
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Authors
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