Influence of 17β-estradiol treatment on the expression of NF-κB in complete hydatidiform mole culture

Background: genetic evidence has established a role of nuclear factor kappa b (nf-κb) signaling in oncogenesis. however, activity of nf-κb in complete hydatidiform mole (chm) cell culture under 17β-estradiol (e2) treatment is not yet known. recently, a positive cross-talk between estrogen receptor (er) and nf-κb to promote survival and progress of cancer cells to a more aggressive phenotype was established. in the present study, we examined the influence of e2 treatment on the nf-κb expression in chm’s culture.methods: this experimental study measured the expression of nf-κb in chm culture treated with e2: 10, 100, 300, 600, and 1000 pg/ml and without e2. imunohistochemistry staining was used to assess the expression of nf-κb. microphotographs were taken using 400x magnification. adobe photoshop cs2 was used to assess the nf-κb expression in cell nucleus. the lower the color intensity of cell rgbbv, is the higher the expression of nf-κb in cells. anova test was performed to compare the expression of nf-κb.results: nf-κb expression as indicated by color intensity in control group was 114.84 ± 9.02. nf-κb expression in e2 treatment groups were respectively: e2 10 pg/ml: 106.30 ± 13.95; e2 100 pg/ml: 82.47 ± 4.72; e2 300 pg/ml: 82.24 ± 2.67; e2 600 pg/ml: 69.05 ± 6.47; e2 1000 pg/ml: 68.49 ± 2.37. there was progressive decline in color intensity of cells with e2 treatment indicating the increase expression of nf-κb. significant differences with the control group occurred in doses of e2 100, 300, 600, dan 1000 pg/ml.conclusion: treatment of chm trophoblast culture with escalating doses of e2 was associated with the increase of nf-κb expression in a dose dependent manner.