Effect of hypoxia-inducible factor-1α induction by CoCl2 on breast cancer cells survival: influence of cytochrome-c and survivin

Background: tumor tissue usually became hypoxic due to disruption of oxygen supply. adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (hif-1α). hif-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. in this study we wanted to analyze the role of hif-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation.methods: breast cancer cell lines t47d were induced by cocl2 then harvested to analyze the expression of hif-1α, protein cytochrome-c, mrna survivin and cell viabilities. results: hif-1α induction by cocl2 causes the increase of protein and mrna of hif-1α, cytochrome-c protein, and survivin mrna, but does not cause the changes in cell viability. conclusion: hif-1α induction have no effects on breast cancer cell line t47d viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.