Increased cell viability and proliferation in post-hypoxic hippocampal tissue culture treated with Acalypha indica root extract

Background: this research was done to study the influence of acalypha indica linn root extract towards relative cell viability and proliferation as parameters of neurogenesis in post-hypoxic hippocampal tissue culture. methods experimental in vitro study using 24 primary neuronal cell cultures obtained from adult sprague dawley rat exposed to hypoxia with 5% o2/5% co2/n2 balance gas for 24 hours. post-hypoxia, acalypha indica linn root extract was added at doses of 10, 15, and 20 mg/ml to 3 treatment groups. no treatment was given to the control group. each group consists of 6 samples. after 90 hours of incubation, relative cell viability was measured by using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) examination, and cell proliferation was measured by using 5-bromo2’-deoxy-uridine (brdu) for cell proliferation. data was analyzed using one way anova parametric tests, then further analyzed with post-hoc analysis. results: the relative cell viability of rat hippocampal tissue culture treated with acalypha indica linn root extract with dose of 10, 15, and 20 mg/ml was significantly higher than control (176.95%, 220.62%, and 386.02% vs. 100%). cell proliferation of rat hippocampal tissue culture treated with acalypha indica linn root extract with dose of 10, 15, and 20 mg/ml was signifi cantly higher than control (0.132, 0.117, 0.114 vs 0.096). conclusion: acalypha indica linn root extract with doses of 10, 15, and 20 mg/ml can increase relative cell viability and proliferation in post-hypoxic hippocampal tissue culture. (med j indones 2011; 20:94-9)