rapid homemade reverse transcriptase pcr detection and phylogenetic analysis for sars-cov-2 based on e and m genes

Background & objective: coronavirus disease 2019 spreads worldwide and needs detection systems capable of rapid diagnostic of this virus (sars-cov-2). the aim of this study is to design the homemade rt-pcr method for the detection and phylogenetic analysis of this virus. material & methods: the genes selected for diagnosis were e and m genes for this virus. pcr product was cloned in ptz57r/t plasmid for preparation of positive control. in order to determine the sensitivity of this molecular method, the genes mentioned in the clone ptz57r/t vector and the limit of detection (lod) the genes were determined and phylogenetic analysis was performed using partial e and m gene sequences. results: pcr product was observed for e and m genes 156 and 547 bp on the agarose gel. the lod of the e and m gene was 60 and 82 copies. there was also a positive response to the samples of patients who were positive by other methods. conclusions: since this virus is considered to be the cause of a pandemic in different countries all over the world, the present study is very important as a method of rapid and low-cost molecular diagnosis for monitoring this virus. phylogenetic analysis is necessary for epidemiological studies for the control and prevention of the disease.