The Impact of Maternal Age on Chromosomal Aneuploidy, Blastocyst Quality, and Pregnancy Outcomes During Intracytoplasmic Sperm Injection Cycles

Background & objective: assisted reproduction techniques and preimplantation genetic testing (pgt) for aneuploidies help infertile couples achieve a healthy live birth. the objective of this study was to investigate if there is a correlation between chromosomal aneuploidy and maternal age. materials & methods: we used 277 embryos with 6-8 cells and graded a derived from intracytoplasmic sperm injection cycles. there were two subgroups: fresh-pgt cycles (n = 38) and frozen embryo transfer (fet) cycles (n = 38). the pgt cycles results were evaluated by fluorescence in situ hybridization and polymerase chain reaction assays. couples (n = 76) with pgt on the third day were classified into four maternal age groups: ≤35 years (n = 35), 36-40 years (n = 24), 41-45 years (n = 11), and ≥46 years (n = 6). the rate of aneuploidy, blastocyst quality, and pregnancy was assessed in fet and fresh-pgt cycle. results: aneuploidy rate in 13, 18, 21, x, and y chromosomes in fet and fresh-pgt cycles are not significantly related to maternal age (p = 0.1). significant differences were found in a decreased chemical pregnancy (p = 0.001), clinical pregnancy (p = 0.001), ongoing pregnancy (p = 0.001), and live birth rate (p = 0.001) among both groups. decreased blastocyst rate increased maternal age in fresh pgt and fet pgt (p = 0.02). early and clinical pregnancy loss was not related significantly to maternal age. conclusion: after evaluating cycle characteristics, an association was found between maternal age and declined pregnancy outcomes in embryos. there was a significant relationship between increasing female age with decreasing blastocyst rate.