the effect of endurance training and l-carnitine supplementation on gene expression of hepatic enzymes (ast,alt,alp) in wistar male rats toxicated by boldenone

Background objective: the aim of this study was to investigate the effect of endurance training and lcarnitine supplementation on gene expression of hepatic enzymes (ast, alt, alp) in wistar male rats toxicated by boldenone.materials methods: in this experimental study, 30 male wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, notreatment, boldenone (5mg/kg), lcarnitine and aerobic training lcarnitine. the endurance moderate intensity training program (5055% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. boldenone injection once a week, on an appointed day, and in the hamstring was conducted in depth. after anesthesia, autopsy was performed and the liver was isolated. the hepatic enzymes gene expression in the samples were measured by real time pcr. data were analyzed by ttest, oneway anova and post hoc scheffe at the significant level p<0.05.results: the results showed that there was a significant difference between the mean expression of liver enzymes (ast, alt, alp) in male wistar rats in different groups (p<0.001). the changes in liver enzymes gene expression (ast, alt, alp) in lcarnitine and traininglcarnitine groups were significantly lower than the boldenone group (p<0.001).conclusion: according to the findings of this study, supplementation of lcarnitine with regular aerobic training reduces liver damage induced by anabolic androgenic steroids. keywords:

The Effect of Endurance Training and L-Carnitine Supplementation on Gene Expression of Hepatic Enzymes (AST,ALT,ALP) in Wistar Male Rats Toxicated by Boldenone

Background Objective: The aim of this study was to investigate the effect of endurance training and Lcarnitine supplementation on gene expression of hepatic enzymes (AST, ALT, ALP) in Wistar male rats toxicated by Boldenone.Materials Methods: In this experimental study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, notreatment, boldenone (5mg/kg), Lcarnitine and aerobic training Lcarnitine. The endurance moderate intensity training program (5055% of maximal oxygen consumption) was performed for 6 weeks and 5 times a week. Boldenone injection once a week, on an appointed day, and in the hamstring was conducted in depth. After anesthesia, autopsy was performed and the liver was isolated. The hepatic enzymes gene expression in the samples were measured by Real Time PCR. Data were analyzed by ttest, Oneway ANOVA and post hoc Scheffe at the significant level P<0.05.Results: The results showed that there was a significant difference between the mean expression of liver enzymes (AST, ALT, ALP) in male Wistar rats in different groups (P<0.001). The changes in liver enzymes gene expression (AST, ALT, ALP) in Lcarnitine and TrainingLcarnitine groups were significantly lower than the boldenone group (P<0.001).Conclusion: According to the findings of this study, supplementation of Lcarnitine with regular aerobic training reduces liver damage induced by anabolic androgenic steroids. Keywords: