A New Method for Multiple Sperm Cells Tracking

Motion analysis or quality assessment of human sperm cell is great important for clinical applications of male infertility. sperm trackingis quite complex due to cell collision, occlusion and missed detection. the aim of this study is simultaneous tracking of multiple humansperm cells. in the first step in this research, the frame difference algorithm is used for background subtraction. there are somelimitations to select an appropriate threshold value since the output accuracy is strongly dependent on the selected threshold value.to eliminate this dependency, we propose an improved non linear diffusion filtering in the time domain. non linear diffusion filteringis a smoothing and noise removing approach that can preserve edges in images. many sperms that move with different speeds indifferent directions eventually coincide. for multiple tracking over time, an optimal matching strategy is introduced that is based on theoptimization of a new cost function. a hungarian search method is utilized to obtain the best matching for all possible candidates. theresults show nearly 3.24% frame based error in dataset of videos that contain more than 1 and less than 10 sperm cells. hence theaccuracy rate was 96.76%. these results indicate the validity of the proposed algorithm to perform multiple sperms tracking.