A New Approach for Designing a Potentially Vaccine Candidate against Urinary Tract Infection by Using Protein Display on Lactobacillus Surface

Background: the prevalence of urinary tract infection (uti) is really high in the world. escherichia coli is a major agent of uti. one of the strategies for decreasing uti infections is vaccine development. as the attachment is a really important stage in colonization and infection, attachment inhibition has an applied strategy. fimh protein is a major factor during bacterial colonization in urinary tract and could be used as a vaccine. thus, it was considered in this research as a candidate antigen. methods: the sequences of fimh and acma genes were used for designing a synthetic gene. it was cloned to pet23a expression vector and transformed to e. coli (de3) origami. to confirm the expression of recombinant protein, sds-page and western blotting methods were used. subsequently, recombinant protein was purified. on the other hand, lactobacillus reuteri was cultured and mixed with fimh / acma recombinant protein. the rate of protein localization on lactobacillus surface was assessed using elisa method. results: it was showed that the recombinant protein was expressed in e. coli (de3) origami and purified by affinity chromatography. moreover, this protein could be localized on lactobacillus surface by 5 days. conclusion: in current study, a fusion recombinant protein was prepared and displayed on l. reuteri surface. this strain could be used for animal experiment as a competitor against uropathogenic e. coli (upec). using manipulated probiotics strains instead of antibiotic therapy could decrease the antibiotic consumption and reduce multi-drug resistant strains.