frequent evaluation of herpes simplex virus type 2 in women with genital herpes by realtime pcr

Background: herpes simplex type 2 is a common infection worldwide. this disease is common in both developed and developing countries. early detection of infection is very important to reduce the risk of infection. real-time reliable pcr is a very sensitive and specific method that can be used as the best marker in determining the therapeutic effect by identifying a viral genome in an individual. the prevalence of herpes simplex virus type 2 in women with genital herpes was evaluated by real time pcr method. methods: from january 1999 to march 2010, 45 samples of vaginal swabs and cervix of women with genital herpes were examined for hsv virus dna detection using real time pcr. results: the mean age of the patients was 35.9 + 5.9. the percentage of positive cases of herpes simplex virus type 2 in the studied women was 22.2% and the history of infection with hpv was 33.3% vs. 12.5%. = 0.094 which was significant. conclusion: clinical specimens of vaginal swabs from genital herpes caused by herpes simplex virus 2 can be quantitatively analyzed instead of nucleic acid extraction and amplification by pcr.