Mouse spontaneous mammary adenocarcinoma as a suitable model of breast cancer in Iran

Background: breast cancer is a complicated disease and has various clinical manifestations and prognosis. the aim of this study is to make a breast cancer model suitable for basic, preclinical and clinical studies. methods: five virgin bulb/c mice aged 6 weeks and spontaneous murine mammary adenocarcinoma cells (mmac) were used. after anesthesia induction, 1-3 million of mmacs were subcutaneously injected into the mice’s right flank. four weeks after the tumor induction, tumor size in both length and width were weekly measured using caliper for 4 weeks. histopathology studies were done 2 months later by scraff-boom-richardson grading. results: visible tumors were appeared after 4 weeks. take-rate of all cells was nearly 100%. tumor sizes reached 0.6±0.1 mm3, 1.1±0.3, 1.68±0.5 and 3±0.7 cm3 with at fifth, sixth, seventh and eighth weeks respectively. the histopathological studies using h & e method showed invasive ductal carcinoma, grade ii/iii. conclusion: elucidation of the molecular mechanisms of breast cancer progression and metastasis has extremely gained from mouse models in which some stages of tumor progression are recapitulated