Effect of nonsurgical periodontal therapy on the level of salivary antioxidants in patients with generalized moderate-to-severe chronic periodontitis

Background. in the course of periodontal diseases, polymorphonuclear leukocytes (pmns) produce oxidative agents andfree radicals, thus triggering oxidant-antioxidant disequilibrium in the saliva. due to the reduction of antioxidant protectiveeffect, oxidative stress is induced, destroying periodontal tissues. this study aimed to investigate the consequences of thenon-surgical phase of periodontal therapy on the level ofsalivary antioxidantsin patients with generalized moderate-to-severechronic periodontitis.methods. un-stimulated salivary samples were collected from 43 patients with generalized moderate-to-severe chronic periodontitis for 5 minutes. clinical parameters, including clinical attachment loss (cal), bleeding on probing (bop) and pocketdepth (pd), were recorded in each tooth and subsequently, scaling and root planing (srp) was carried out. after four weeks,salivary samples were collected once again, and the above-mentioned clinical parameters were recorded. following centrifugation and freezing at a temperature of -80°c, salivary samples were examined simultaneously in a single day, and the levelof their antioxidants was measured with ferric reducing ability of plasma (frap) method using a spectrophotometer.results. the concentration of salivary antioxidants significantly increased four weeks following the non-surgical periodontaltherapy (p<0.0001). moreover, the clinical parameters of cal, bop and pd showed a significant decrease in 4 weeks aswell (p<0.0001).conclusion. the level of salivary antioxidants in patients with generalized moderate to severe chronic periodontitis significantly increased after etiotropic periodontal therapy, indicating the possible beneficial influence of periodontal therapy onthe level of salivary antioxidants in patients suffering from periodontitis.