association between thrombophilic gene polymor-phisms and recurrent pregnancy loss in iranian women

Background: recurrent pregnancy loss (rpl) is a common problem among couples, and acquired thrombophilia is the well-known etiology of rpl. the aim of this study was to establish the association between inherited thrombophilic gene polymorphisms and rpl. methods: this case-control study was conducted on 50 women with unexplained rpl and 50 parous women with no history of miscarriage (age range: 17–48 years). the data were collected during 2013–2015 in sarem hospital, tehran, iran. genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) for glycoprotein iiia (pla1/pla2), kdr (q472h), and β-fibrinogen (-455g/a); tetra-primer amplification refractory mutation system (arms)-pcr for glycoprotein ia (807c/t) and vascular endothelial growth factor (vegf) (2578c/a), and ins/del pcr for angiotensin i-converting enzyme (ace) (intron 16 i/d). the association between the frequency of the genotypes and rpl was determined by chi-square and fisher’s exact tests. results: the results of the present study revealed a significant relationship between glycoprotein ia (807c/t), vegf (2578c/a), and ace (intron 16 i/d) polymorphisms and rpl (p=0.00, 0.02, and 0.00, respectively). in contrast, no relationship was observed between β-fibrinogen (-455g/a), kdr (q472h), and glycoprotein iiia (pla1/pla2) polymorphisms and increased risk of rpl (p>0.05). conclusion: this study demonstrated that glycoprotein ia (807c/t), vegf (2578c/a), and ace (intron 16 i/d) polymorphisms may be a risk factor for the women with a history of rpl.