association of apoa1-75g/a and +83c/t polymorphic variation with acute coronary syndrome patients in kashmir (india)

Introduction: acute coronary syndrome (acs) comes under the ambit of cardiovascular disease. apoa-1 gene plays a vital role in lipid metabolism and has been observed to have plausible role in acs. this cross sectional case-control study was conducted to evaluate association between apoa1-75g/a (rs1799837), +83c/t (rs5069) genotypes and risk for acs. methods: the current case-control study that included confirmed 90 acs cases and 150 healthy controls were genotyped for apoa1-75 g/a and +83 c/t by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflf) method. results: apoa1-75g/a distribution of genotypes/alleles among cases and controls was seen proportionally same with no association to acs (p = 0.5). apoa1+83 c/t variants showed protective effect with acs where variant tt genotype presented more in controls (12%) than cases (1.6%) (p = 0.004) and likewise variant ‘t’ allele was found more in controls than acs cases (9.4% vs.28.5% respectively: p < 0.05). further, significantly high difference of ct genotype was seen among cases and controls 15% vs. 33% respectively (p = 0.002). the overall distribution of different haplotypes showed a marked difference in gt when compared with gc between cases and controls (p = 0.0001). conclusion: the study shows that tt genotype and variant t allele of apoa1 +83 c/t depicted a protective role with respect to acs whereas apoa1-75g>ashowed no relation. haplotype gt was observed to significantly over-represent in controls with its protective effect in acs as against wild type haplotype gc.