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oxidized cholesterol exacerbates toll-like receptor 4 expression and activity in the hearts of rats with myocardial infarction
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نویسنده
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khorrami arash ,ziaee mojtaba ,rameshrad maryam ,nakhlband ailar ,maleki-dizaji nasrin ,garjani alireza
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منبع
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journal of cardiovascular and thoracic research - 2020 - دوره : 12 - شماره : 1 - صفحه:43 -50
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چکیده
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Introduction: the present study examined the effects of high cholesterol and high oxidized-cholesterol diets on the myocardial expression of tlr4 and pro-inflammatory cytokine in rats. methods: male wistar rats were allocated into 6 groups and fed with a normal diet, cholesterol, and oxidized-cholesterol rich diets with or without isoproterenol-induced myocardial infarction. tlr4 and myd 88 expression and levels tumor necrosis factor alpha (tnf-α) and interleukin 6 (il-6) were measured in the heart and serum. results: oxidized cholesterol-fed animals had higher serum levels of oxidized low-density lipoprotein (ldl) (263 ± 13 ng/dl) than the cholesterol-fed animals (98 ± 8 ng/dl; p < 0.001). a high level of oxidized-ldl caused fibrotic cell formation and enhanced neutrophil infiltration in the absence of mi. both cholesterol and oxidized-cholesterol upregulated tlr4 mrna expression and increased tnf-α and il-6 production in the hearts of rats with mi. in rats fed with oxidized-cholesterol the serum and myocardial levels of tnf-α (653 ± 42 pg/ml, 1375 ± 121 pg/100 mg, respectively) were higher than mi group (358±24 pg/ml, p < 0.001 and 885 ± 56 pg/100 mg, p < 0.01). a significant correlation was seen between tlr4 expression and infarct size. conclusion: these findings suggest that cardiac tlr4 is preferentially upregulated by oxidized cholesterol in rats. oxidized cholesterol may have a critical role in cardiac toxicity in the absence of pathological conditions.
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کلیدواژه
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oxidized cholesterol ,toll-like receptor 4 ,inflammation ,myocardial infarction ,cytokine ,rats
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آدرس
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maragheh university of medical sciences, medicinal plants research center, iran. tabriz university of medical sciences, faculty of pharmacy, department of pharmacology, iran, maragheh university of medical sciences, medicinal plants research center, iran. tabriz university of medical sciences, cardiovascular research center, iran, north khorasan university of medical sciences, natural products and medicinal plants research center, iran. tabriz university of medical sciences, faculty of pharmacy, department of pharmacology, iran, tabriz university of medical sciences, research center for pharmaceutical nanotechnology, faculty of pharmacy, department of pharmacology, iran, tabriz university of medical sciences, faculty of pharmacy, department of pharmacology, iran, tabriz university of medical sciences, faculty of pharmacy, department of pharmacology, iran
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پست الکترونیکی
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garjania2002@yahoo.com
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Authors
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