Determination of enrofloxacin residue in chicken eggs using FPT and ELISA methods

Due to the indiscriminate use of antibiotics in poultry industry and non-compliance withdrawal times. the aim of present study is detection of enrofloxacin residue in chicken eggs from northwest regions of iran (tabriz, urmia and ardabil cities) using four plate test (fpt) and elisa methods. at the first time, one hundred and fifty egg chickens were collected randomly from market of northwest regions of iran and antibiotics residue was determined by ftp method. the results of fpt revealed that up to 60.66% of the samples (91/150) were contaminated with the antibiotics residue; the egg samples of ardebil (42.85%) and tabriz (27.27%) had the highest and lowest percentage of contamination. the elisa assay showed that out of 91 positive samples in fpt, 78 samples (85.71%) were positive for enrofloxacin. elisa analyses demonstrated that the maximum levels of (56.17 ppb) and the highest contamination rate (43.58%) were observed in ardabil egg samples. concluding, the findings of this study suggest that the indiscriminate use and non-compliance withdrawal times of enrofloxacin in poultry farms of northwest regions of iran. the raising awareness of poultry breeders about the risks of antibiotic residues in food products of animal origin, along with the exact compliance withdrawal times can be very helpful.