synthesis, characterization and cytotoxic studies of novel 1,2,4-triazole-azomethine conjugates

A series of 1,2,4-triazole-schiff hybrids were synthesized and characterized by mass spectrometry, ftir and nmr spectroscopy. the compounds were screened for anticancer activity against human breast cancer cell line (mcf-7 and t47d) and human cervical cancer cell line (hela). the result indicates that newly synthesized compounds exhibit cytotoxicity to all cell lines studied. in particular, mcf-7 cells were shown to be more sensitive with ec50 < 50 μm/ml with the exception of compound t2. interestingly, t6 produced more high cytotoxicity on mcf-7 cells (< 10 μm/ml), which is comparable to cisplatin ec50 13.10 μm/ml. to further investigate the mode of cell death, early and late apoptosis studies were done on mcf-7 cells. the externalization of phosphatidylserine and dna fragmentation supports the apoptosis as the major mode of cell death induced by derivatives on mcf-7 cells.