>
Fa   |   Ar   |   En
   simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography–fluorescence detection  
   
نویسنده mehdinia ali ,rostami simindokht ,dadkhah sahar ,fumani neda sheijooni
منبع journal of the iranian chemical society - 2017 - دوره : 14 - شماره : 10 - صفحه:2135 -2142
چکیده    Simultaneous analysis of homotaurine and its homologous, taurine, is a highly challenging issue, especially in matrices they exist simultaneously. a simple precolumn derivatization procedure combined with high-performance liquid chromatography–fluorescence detection was developed for simultaneous determination of homotaurine and taurine in marine macro-algae. the analytes were derivated with o-phthalaldehyde at an ambient temperature and alkaline medium. calibration curves were linear in the ranges of 50–2500 µg l−1 for homotaurine and 100–2500 µg l−1 for taurine with the coefficients of determination higher than 0.998. limits of detection of homotaurine and taurine were 15 and 30 µg l−1, respectively. intraday (n = 6) and inter-day (n = 4) precisions of the method were satisfactory with relative standard deviations less than 6.0%. good recoveries (>94%) were acquired by the method for extraction of homotaurine and taurine from algae matrices. liquid chromatography–mass spectrometry was also used to confirm detection of the analytes in algae samples. the data suggest that the method was successfully applied to simultaneous determination of homotaurine and taurine in algae samples.
کلیدواژه homotaurine ,taurine ,precolumn derivatization ,hplc ,fluorescence detection ,marine algae
آدرس iranian national institute for oceanography and atmospheric science, ocean sciences research center, department of marine living science, iran, k. n. toosi university of technology, faculty of science, department of chemistry, iran, k. n. toosi university of technology, faculty of science, department of chemistry, iran, iranian national institute for oceanography and atmospheric science, ocean sciences research center, department of marine living science, iran
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved