>
Fa   |   Ar   |   En
   study of urease inhibitory activity by medicinal plants extract based on new catalyst for berthelot reaction and taguchi experimental design  
   
نویسنده share neda babazadeh ,salehabadi hafezeh ,zeidabadi farnoush ,souri effat ,amanlou massoud
منبع journal of the iranian chemical society - 2018 - دوره : 15 - شماره : 3 - صفحه:547 -554
چکیده    The berthelot reaction is a well-established colorimetric method for determination of ammonia. in this work, the effects of different bivalent ions (ba2+, cd2+, co2+, cu2+, fe2+, hg2+, mg2+, mn2+, ni2+, and pb2+) were studied as catalyst on the berthelot reaction efficiency. cucl2 was generally found as the best catalyst that provides a rapid and stable blue indophenol color. the taguchi experimental design methodology has been applied to find optimum conditions. four factors including temperature, ph, reaction time, and cucl2 concentration at five levels were considered to achieve optimum conditions. blue indophenol color stability for 40 min, and linearity response up to 20 mm of ammonium sulfate were achieved by further validation experiments. limit of detection and quantification for this approach was 0.15 and 0.5 mm, respectively. inhibitory activity of three traditional medicinal plants extract (citrus aurantifolia, laurus nobilis, and zingiber officinale) was evaluated against jack bean urease activity by berthelot reaction in the presence of cucl2 as catalyst, and results were compared with traditional berthelot reaction.
کلیدواژه berthelot reaction ,catalyst ,taguchi methodology ,urease ,inhibitor ,medicinal plants
آدرس tehran university of medical sciences, faculty of pharmacy and drug design and development research center, department of medicinal chemistry, iran. islamic azad university, south tehran branch, department of engineering, iran, tehran university of medical sciences, faculty of pharmacy and drug design and development research center, department of medicinal chemistry, iran, tehran university of medical sciences, faculty of pharmacy and drug design and development research center, department of medicinal chemistry, iran, tehran university of medical sciences, faculty of pharmacy and drug design and development research center, department of medicinal chemistry, iran, tehran university of medical sciences, faculty of pharmacy and drug design and development research center, department of medicinal chemistry, iran
 
     
   
Authors
  
 
 

Copyright 2023
Islamic World Science Citation Center
All Rights Reserved