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   a validated chiral lc method for the enantiomeric separation of at13148 on polysaccharide-based chiral stationary phase  
   
نویسنده xia yong ,liu zhihao ,huang yong ,huang xiaojun ,liao mengya ,zhang yiwen ,ma xuelei
منبع journal of the iranian chemical society - 2018 - دوره : 15 - شماره : 3 - صفحه:711 -717
چکیده    A sensitive and accurate lc method for the determination of at13148 enantiomeric purity has been developed and validated. baseline separation with a resolution higher than 1.8 was accomplished within 15 min using a chiralpak ad-h column (250 × 4.6 mm; particle size 5 μm) and n-hexane: 2-propanol: diethylamine (85:15:0.1, v/v) as mobile phase at a flow rate of 1 ml min−1. eluted analytes were monitored by uv absorption at 254 nm. the effects of mobile phase components, temperature and flow rate on enantiomeric selectivity and resolution of enantiomers were investigated. calibration curves were plotted within the concentration range between 7 and 500 μg ml−1 (n = 11), and the recoveries between 98.24 and 100.99% were obtained, with relative standard deviation lower than 1.32%. lod and loq for at13148 were 2.46 and 7.38 μg ml−1 and for its enantiomer were 2.54 and 7.49 μg ml−1, respectively. it was demonstrated that the developed method was accurate, robust and sensitive for the determination of enantiomeric purity of at13148, especially for the analysis of bulk samples.
کلیدواژه column liquid chromatography ,chiral separation ,enantiomeric purity ,at13148
آدرس sichuan university and collaborative innovation center, cancer center, west china hospital, china, sichuan university and collaborative innovation center, cancer center, west china hospital, china, sichuan taihuatang pharmaceutical co., section 4, china, second clinical medical college of jinan university (shenzhen people’s hospital), department of hepatobiliary and pancreas surgery, china, sichuan nursing vocational college, china, sichuan university and collaborative innovation center, cancer center, west china hospital, china, sichuan university and collaborative innovation center, cancer center, west china hospital, china
 
     
   
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