design, synthesis, structure elucidation and in vitro antiviral and antimicrobial evaluation

In this study, we described the synthesis of the derivatives of thiosemicarbazide, dicarboximide, 1,2,4-triazole-5-thione and 4-oxo-1,3-thiazolidine. two different dicarboxylic acid anhydrides reacted with 4-substituted-3-thiosemicarbazide, and derivatives of thiosemicarbazide and dicarboximide were obtained. next, cyclization reaction of dicarboximide derivatives in alkaline media was used to prepare 1,2,4-triazole-5-thione. the 4-oxo-1,3-thiazolidine was synthesized by the reaction of dicarboximide with ethyl bromoacetate. all obtained derivatives were analysed by 1h and 13c nmr spectra, and for one compound, the x-ray crystallography was done. antimicrobial, antiviral and in vitro evaluations of cytotoxicity were examined. according to the preliminary antiviral screening, compounds 3 and 4 presented the antiviral activity against hsv-1 and cvb3. additionally, compound 3 shows selective in vitro toxic effect against human epithelial cells fadu, without affecting normal animal cell line (vero). the same derivatives 3 and 4 also displayed a wide spectrum of antimicrobial activity against reference microorganisms and indicated both antibacterial and antifungal potential activities.