A Case Study to Evaluate the Role of Basiluses in Producing Biosurfactant and the Feasibility of MEOR

Bibi hakimeh oilfield consists of more than 145 oil producing wells. its oligomiocene asmari reservoir is dominantly made of limestone . the act of a reverse fault on the north flank of bibi-hakimeh field caused a signinficant thickness reduction in gachsaran formation in the way that in some drilled wells members no.2,3,4,5,and 6 of gachsaran cap rock have been totally eliminated which causes locating samari reservoir in a shallower level and therefor lower reservoir temperature in the north flank already have made the reservoir suitable for a microbiological enhanced recovery.the long term production of this reservoir caused a signigicant reservoir pressure drop therefore; the recovery has been performed using basilus with ex-situ method. in this case study the feasibility of surfactants production in several oil wells has been accomplished . a high temperature resistant basilus has been selected to evaluate the production. ability of biosurfactant. this bacterium has been elected after performing all morphological , biochemical and genetic studies. this bacteria shows a good resistance against the temperature in such manner that its emulsification, surface tension and inter surface tension abilities remains with no change after 15 min autoclave process under 120 c. in the next step, the temperature, ph, carbon, n2 and other factors have been. optimized for biosurfactant production. considering the lithology of the reservoir using this type of bacterium can be a good way to produce lipopithidic biosurfactant by ex-situ method in asmari the enhanced oil recovery . the basilus which have good resistance against temperature and act well in pressurized environments can be considered as a good candidate for tertiary enhanced oil recovery process. the best method to produce basilus in b5 is the formislink method