photosensitizing activity of nanoparticles of poly (2-amino phenol)/gold for intensified doxorubicin therapeutic effect on melanoma cancer cells under synergism effect of 808-nm light

Background: photothermal therapy (ptt) is one of the effective and non-invasive strategies which hold great promise for improving the treatment of cancer cells. ptt is based on activating a photosensitizer by infrared light irradiation and producing heat and reactive species and apoptosis in the tumor area.objective: the aim of this study was to investigate the effect of photothermal/chemotherapy on melanoma cancer cells using poly (2-amino phenol)/gold (p2ao/aunps) and doxorubicin (dox).material and methods: in this experimental study, nanoparticles of p2ao/aunps were synthesized, and their mixture with dox was applied as a photosensitizer for photothermal/chemotherapy of a c540 (b16-f10) melanoma cell line.results: p2ao/aunps generated heat and cytotoxic responsive oxygen species (ros) upon 808-nm light irradiation with simultaneous intensifying dox therapeutic effect under domination of synergism effects between light irradiation, p2ao/aunps, and doxorubicin. cell treatment with both p2ao/aunps and dox resulted in a considerable increase in necroptotic cells to 61% with a significant decrease in the living cells (39%).  conclusion: p2ao/aunps provided a platform for light absorption and intensifying dox therapeutic effect. this study approved the applicability of a new photothermal/chemotherapy by domination of synergistic effects attained by combination of laser light, p2ao, aunps, and dox.