a β-amyloid_(1-42) biosensor based on molecularly imprinted poly-pyrrole for early diagnosis of alzheimer’s disease

Background: alzheimer’s disease (ad) is a common form of dementia, characterized by production and deposition of β-amyloid peptide in the brain. thus, β-amyloid peptide is a potentially promising biomarker used to diagnose and monitor the progression of ad. objective: the study aims to develop a biosensor based on a molecularly imprinted poly-pyrrole for detection of β-amyloid. material and methods: in this experimental study, an imprinted poly-pyrrole was employed as an artificial receptor synthesized by electro-polymerization of pyrrole on screen-printed carbon electrodes in the presence of β-amyloid. β-amyloid acts as a molecular template within the polymer. the biosensor was evaluated by cyclic voltammetry using ferro/ferricyanide marker. the parameters influencing the biosensor performance, including electro-polymerization cycle umbers and β-amyloid binding time were optimized to achieve the best biosensor sensitivity. results: the β-amyloid binding affinity with the biosensor surface was evaluated by the freundlich isotherm, and freundlich constant and exponent were obtained as 0.22 ng ml-1 and 10.60, respectively. the biosensor demonstrated a detection limit of 1.2 pg ml-1. the biosensor was applied for β-amyloid determination in artificial cerebrospinal fluid. conclusion: the biosensor is applicable for early alzheimer’s disease detection.