combined effect of iodine contrast media, cisplatin and external beam radiotherapy on anaplastic thyroid cancer cells

Introduction: the current study investigated the combination of high z atoms(iodine-, platinium-based drugs) with using low energy irradiation (120kvp) in anaplasticthyroid cancer cells.material and methods: for this purpose, eight groups were designed: control(cnt), different concentrations of iodine contrast media (icm), irradiation with variousdoses, cis-platin (cddp) with different concentrations, (icm + cddp), (icm +rad), (cddp + rad) and (icm + cddp + rad). the viability was measured bymtt and colony assay. in mtt assay, the viability of 8305c cells rad (2 gy)+icm(10mg/ml) group was significantly lower than those treated with rad or icm alone.cddp +icm+rad group significantly decreased the viability. in colony assay, cellsin icm + rad (2 gy) group reduced the number of colonies more significant thanrad group. the difference of colony forming ability between cddp and cddp+ rad (2 gy) was significant. the difference of icm + cddp + rad (2 gy) andcddp +rad (2 gy) group was significant. all data were statistically analysed usingone-way analysis of variance (anova) followed by chafe’s multi-comparisonstests. all data were presented as mean ± standard deviation (sd) and analysed usingstatistical package for social sciences (spss 16). significance was considered to bep<0.05.results: in mtt assay, the viability of 8305c cells rad (2 gy) + icm (10mg/ml) group was significantly lower than those treated with rad or icm alone. cddp+ icm + rad group significantly decreased the viability. in colony assay, cells inicm + rad (2 gy) group reduced the number of colonies more significantly thanrad group. the difference of colony forming ability between cddp and cddp+ rad (2 gy) was significant. the difference of icm + cddp + rad (2 gy) andcddp + rad (2 gy) group was significant.conclusion: exposure of atc to icm in the presence of cddp increases tissuex-rays absorbance by auger electrons and photo electrons leading to more fatal effectsagainst the tumour.