an aptamer-based biosensor for troponin i detection in diagnosis of myocardial infarction

Background:acute myocardial infarction (mi) accounts for one third of deaths. cardiac troponin i (tni) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of mi. objective: in this study, a molecular method is introduced to early diagnosis of mi by rapid detection of tni. materials and methods: the detection method was based on electrochemical aptasensing, being developed using different methods and evaluation steps. a gold electrode was used as a transducer to successful immobilize 76base aptamer to fabricate a tni biosensor. results: the designed aptasensor could detect tni in a range of 0.03 to 2.0 ng ml^-1 without using any label, pre-concentration or amplification steps. the limit of detection was attained as 10 pg ml^-1 without significant trouble of interfering species. the tni biosensor demonestrated a stable, regenerative and reproducible function. 89 human samples were used to evaluate the performance of the tni biosensor, and it represented 100% and 81%, diagnostic sensitivity and specificity, respectively. conclusion: this aptasensor may be used as an applicable tool in the future of early medical diagnosis of mi.