Analysis of Exon 19 and 39 of ATM Gene in Brain Tumors; Considering the P53 Accumulation in Patients with ATM Alteration

Many studies have been focused on cytogenetic and molecular genetic defects in brain tumors; therefore the role of atm as a tumor suppressor gene in these tumors is poorly considered. in this study mutation analysis of exon 19 and 39 of atm gene and p53 accumulation were investigated by pcr-sscp, sequencing, and flow cytometry . four polymorphisms including d1853n, ivs 38-8 t—c, f858l, p872t were reported for the first time in brain tumors other than medulloblastoma. expression of p53 could be detected in more than 10% of cells in patients affected with meningioma and 4.08% and 3.46% of cells inastrocytoma and chordoma respectively. the present findings could confirm the importance of atm gene alterations in tumor genesis of brain tumors and further investigation is essential.