Investigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch.

Parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of theumbellifera family, is one of the most important medicinal species with high economicvalue. generally, there is limited information about in vitro culture of bunium persicum.in this study, the effect of various media (ms, b5) and combinations of plant growthregulators, naa and 2,4-d alone or together with kin, on callus induction and shootregeneration from hypocotyl explant were investigated. this experiment has been carriedout in a completely randomized design with 30 treatments and 10 replications pertreatment. the results showed significant effects of treatments on regeneration and callusinduction. all the measured traits for ms medium were superior. the highest callusfrequency was observed on the medium containing 0.1 mg l-1 or 1 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-d) as well as 2 mg l-1 2,4-d and 0.5 mg l-1 kinetin. thebest treatment for somatic embryogenesis was the medium containing 2 mg l-1 2,4-d. thebest response for shoot regeneration was observed on the medium supplemented with 1mg l-1 2,4-d. the medium containing 1 mg l-1 2,4-d was the best for callus induction andshoot regeneration simultaneously. the regenerated shoots were rooted on basal medium.