Efficiency of Lignocellulolytic Extracts from Thermotolerant Strain Fomes sp. EUM1: Stability and Digestibility of Agricultural Wastes

Production of lignocellulolytic enzymes by the thermotolerant fomes sp. eum1 was determined in solid cultures using corn stover (cs) as a sole substrate or supplemented with 20 % wheat bran (cs+wb). this supplementation increased (p< 0.05) enzymatic activity per gram of initial dry matter (gdm) for xylanases and cellulases: 160 iu g dm^-1 and 37 iu g dm^-1, respectively; while laccases reached a similar yield (3.3 iu g dm^-1) for both cultures. nevertheless, laccases showed different stability patterns at 39°c and ph 6: half-life time (t½) was doubled in extracts from cs+wb (23.5 h); whereas t½ for the other enzymes from both cultures showed no difference. both extracts by fomes sp. eum1 and a commercial enzymatic product were used on forages: corn stover, (cs), sugarcane bagasse (scb), and alfalfa hay (ah). the fractional rate of gas production (fr; ml g^-1 h^-1) increased (p< 0.05) at 9 hours in cs compared to the sample without enzymes. the use of any enzymes favoured higher maximum gas volume (vm; h^-1) on scb. the in vitro digestibility (ivd) of cs after using the commercial product was 12% higher, while our extracts from cs and cs+wb showed 16 and 21% improvements (p< 0.05), respectively, suggesting a higher specificity of these enzymes produced on the same substrate (cs). in addition to the proven stability, the versatility of extracts from cs and cs+wb was confirmed by the increase in ivd values for scb (up to 100%) in relation to the control without enzymes.