Simulation of Absorption Spectrum of Photosynthetic Pigments of Chlorella Vulgaris B Algae

Absorption spectrum of chlorella vulgaris b algae was reconstructed by simulated the absorption spectrum of the mixture under examination by a linear combination of the absorption spectra of all its constituents. a c30 reversed high performance liquid chromatography equipped with photodiode array detector (hplc-pad) and absorption spectrophotometer were used to separate and identify photosynthetic pigments of chlorella vulgaris b. spectrum-reconstruction method (srcm) was used to calculate concentrations of total carotenoids,chlorophylls a and b. absorption spectrum of the total pigments extracted of chlorella vulgaris b. was reconstructed from the sum of the spectrum of individual extracted pigments taken from (hplc-pad),and compared using normalization with absorption spectrum of the total pigments recorded by spectrophotometer. a total of seven pigments were separated and identified.