Frequency of subgroups of diffuse large B-cell lymphoma by immunohistochemistry

Objective: to determine the frequency of subgroups (germinal centre and non germinal centre) of diffuse large b cell lymphoma (dlbcl) in a tertiary care hospital. materials and methods: it is a descriptive cross sectional study performed in histopathology department,clinical laboratories dr. ziauddin hospital karachi. 62 cases of diffuse large b cell lymphoma were collected during fifteen (15) months period from april 2011 to june 2012. all resected specimens and small biopsies were grossed; sections were processed overnight and stained by hematoxylin and eosin (h&e) for morphologic evaluation. the panel of lymphoid antibodies included cd20,cd79a,cd3,ki-67 & pax5 were used for the diagnosis of dlbcl. then monoclonal antibodies cd 10,bcl-6 and mum-1 were applied for subgrouping of dlbcl. results: in a total of 62 cases of diffuse large b cell lymphoma,27 (44%) cases were categorized as germinal centre like subgroup and 35 (56%) were categorized as non germinal centre like subgroup. patient’s ages ranged from 04 to 95 years. 58% patients were males and 42% were females with male to female ratio1.4:1. 36(58%) cases were extranodal and 26(42%) were nodal lymphoma. non germinal center like subgrpoup was preponderance in both nodal and extranodal lymphoma as 15(58%) and 20(56%) respectively. conclusion: this study reports an increased number of patients of non germinal centre subgroup of diffuse large b cell lymphoma. male to female ratio was 1.4:1. mean age of patient was 45 years. © 2015,liaquat university of medical and health sciences. all rights reserved.