Determination of Aflatoxin M1 Levels in Produced Pasteurized Milk inAhvaz City by Using HPLC

Aflatoxins are one of the most potent toxic substances that occur naturally.nowadays extensive attention has been taken to their existence in food and environment,as there is the possibility of harm to humans following chronic exposure toextremely low levels via food chain. aflatoxin m1 (afm1) is a hepatic carcinogenic metabolitefound in the milk of lactating animals fed with contaminated feed contaminated byaflatoxin b1 (afb1).objectives: this study aimed to determine the levels of afm1 in produced pasteurizedmilk in the ahvaz of city.materials and methods: for this purpose, 100 samples of pasteurized milk from the jamusfactory were analyzed the to determine afm1 content by using an immunoaffinitycolumn for clean-up and high-performance liquid chromatography (hplc) with a c18column, a fluorescence detector (excitation 365 nm, emission 435 nm) and a mobilephase of acetonitrile–water (25:75, v/v) at a flow rate of 1 ml/min.results: afm1 was detected in all 100 samples of pasteurized milk at concentrationsranging from 0.45 to 9.760 ng/l.conclusions: the mean concentration of afm1 in the the pasteurized milk samples was2.7 ng/l, which was below the 50 ng/l, accepted as level of for milk in iran.