a compound from vietnamese acacia pennata (l.) willd inhibits lps-induced inflammatory signals on macrophage

Background:acacia pennata (l.) willd is used as an herbal medicine worldwide. however, the compounds isolated from this plant that have anti-inflammatory properties require further study. objectives: this study isolated compounds with anti-inflammatory and reactive oxygen species (ros) inhibitory properties from a. pennata (l.) willd. methods: the compounds from the stem of vietnamese a. pennata (l.) willd were extracted with ethanol. high-performance liquid chromatography (hplc) was used to analyze the purity of the obtained substances. the substances were analyzed by 1h, 13c- nuclear magnetic resonance (nmr) spectroscopy, distortionless enhancement by polarization transfer (dept), heteronuclear single quantum coherence (hsqc), and heteronuclear multiple bond correlation (hmbc) spectra. the anti-inflammatory ability of compound 3 (arg1) was assessed through the inhibition of pro-inflammatory cytokines using elisa. the effectiveness of inhibiting ros was measured by a specific fluorescent immunostaining technique. the activity of nicotinamide adenine dinucleotide phosphate (nadph) oxidase enzyme was determined using a luminometer, and the expression of phosphorylated p47phox was detected by protein gel electrophoresis in raw 264.7 cells (mouse macrophage cell line). results: the obtained results indicated that arg1 was identified as ginsenoside rg1. in this study, arg1 (40 μg/ml, p < 0.001) inhibited lipopolysaccharide (lps)-mediated secretion of tumor necrosis factor (tnf)-α, as well as interleukin (il)-6 in macrophages. the phosphorylation of p47phox and nadph oxidase was inhibited by arg1 (40 μg/ml, p < 0.01) in lps-stimulated cells. more importantly, ros generated in lps-stimulated cells was also destroyed by arg1 (40 μg/ml). conclusions: collectively, the data showed that arg1 found in the chemical composition of a. pennata (l.) willd was similar to ginsenoside rg1. this is the first result found in a species of the genus acacia. in addition, these data showed that inflammatory cytokines and ros generated by lps in cells were inhibited by arg1.