targeted solid lipid nanoparticles formulation for colon cancer treatment and cytotoxicity assessment using ht29 cell line

Background: several pharmacological effects have been attributed to thymol. however, limitations such as low solubility in water, low bioavailability, and high volatility have limited its use. objectives: the present study aimed to prepare and characterize thymol-loaded solid lipid nanoparticles (slns) to improve the efficacy of thymol. methods: thymol-loaded slns were characterized by atomic force microscopy (afm), differential scanning calorimetry (dsc), and fourier transformed infrared spectroscopy (ft-ir). cytotoxicity study and hemolysis assay were also performed. results: the in vitro drug release showed a sustained manner. also, slns loaded with thymol showed higher cytotoxicity than free thymol, and the hemolysis results indicated the blood biocompatibility of slns. conclusions: as nanocarriers, slns can open a new avenue for improving the efficacy of thymol in cancer treatment.