characteristics and hepatoprotective activity of the curcuma heyneana rhizome extract toward wistar rats induced by ethanol

Background: curcuma heyneana (valeton zijp.) or temu giring has various pharmacological activities. however, its hepatoprotective activity toward ethanol induction has never been carried out. objectives: the objective of this research was to evaluate the hepatoprotective activity of the c. heyneana rhizome extract toward wistar rats induced by ethanol. methods: the research was initiated with the determination of curcuminoid content, total phenolic content, total flavonoid content, and characterization of extract using gas chromatography-mass spectrometry (gc-ms). hepatoprotective activity was tested using the c. heyneana extract at doses of 50, 100, and 150 mg/kg with 5 g/kg ethanol as an inducer. aspartate transaminase (ast), alanine transaminase (alt), liver weight, and macroscopic and microscopic liver were used as parameters. data were analyzed using analysis of variance (anova). results: the curcuminoid content of the extract was 1.18% (w/w). total phenolic content of the c. heyneana extract was 400.37 mg gallic acid equivalent (gae)/g sample, while total flavonoid content was 27.25 mg quercetin equivalent (qe)/g sample. nine compounds were identified in the extract. administration of the extract at doses of 50, 100, and 150 mg/kg kept the liver normal. it was identified macroscopically from the dark red color without any white spot and normal liver weight. furthermore, at doses of 50, 100, and 150 mg/kg, the extract inhibited ast and alt elevation, which was significantly different from the negative control group (p lt; 0.05). the extract also prevented hepatocyte injury that was seen microscopically. conclusions: it can be concluded that the c. heyneana extract at doses of 50, 100, and 150 mg/kg is effective as hepatoprotective in the liver injury induced by ethanol.