efflux <i>mexab</i>-mediated resistance in multidrug and pan-drug resistant strains of <i>pseudomonas aeruginosa</i> isolated from patients with burn and wound infections

Background]the emergence of highly drug resistant <i>pseudomonas aeruginosa</i> in burn wounds is becoming a challenging problem for infection control programs. today, it has been shown that antibiotic resistance in <i>p. aeruginosa</i> is the result of synergism between membrane permeability and multidrug resistance (mdr) efflux pumps.[objectives]the aim of this study was to investigate mdr and pandrug resistance (pdr) in <i>p. aeruginosa</i> and detection of the presence of efflux pump <i>mexab</i> genes by the polymerase chain reaction (pcr) technique.[materials and methods]onehundred and fifty <i>p. aeruginosa</i> were isolated from burn and wound infections. the isolates were confirmed using conventional culture and biochemical tests. antibiotic resistance was evaluated using agar disk diffusion and broth micro dilution tests. for detection of efflux pump <i>mexab</i> genes, the pcr technique with subsequent sequencing was used.[results]in total, 99 strains (66%) were mdr and one strain (0.667%) was pdr as detected by traditional susceptibility tests. the mdr isolates belonged to 53 burn (70.66%) and 46 wound (61.33%) infections. the pdr was only seen in one isolate from a burn strain (1.33%). the pcr technique revealed that all the 99 mdr strains and one pdr strain, contained <i>mexa</i> and <i>mexb</i> genes, representing an exhibitive intrinsic existence of these genes in mdr and pdr of <i>p. aeruginosa</i>.[conclusions]this study represented an increasing rate of mdr <i>p. aeruginosa</i> in burn and wound samples. efflux <i>mexab</i> genes were detected in all mdr and pdr strains. the <i>p. aeruginosa</i> strain isolated from burn cases showed higher drug resistance and pdr resistance was only noted in a burn sample.