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a simple method for extraction and purification of hypericins from st john's wort
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نویسنده
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ramezani zahra ,zamani mitra
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منبع
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jundishapur journal of natural pharmaceutical products - 2017 - دوره : 12 - شماره : 1 - صفحه:1 -4
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چکیده
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Hypericin was extracted from hypericum perforatum l. using a different extraction procedure. highest amounts of hypericins were extracted by ultrasonic methods using methanol: acetone 2:1 as an extracting solvent. hypericin contents of the extract was purified by passing it through 2 series of 70×5 mm glass columns, each filled with 800 mg of 35 - 70 mesh silica gel, followed by elution with meoh: acetone: ch2cl2 (75:10:15). the extent of purification was determined by hplc. the amount of purified hypericins, which was extracted from hypericum perforatum, was 5.105 mg per gram dried plant leaves.
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کلیدواژه
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hypericin ,pseudo-hypericin ,hplc ,ultrasonic assisted extraction ,hypericum perforatum l.
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آدرس
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ahvaz jundishapur university of medical sciences, faculty of pharmacy, department of medicinal chemistry, ایران, azad university, ahvaz branch, faculty of science, science and research center, department of chemistry, ایران
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Authors
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