investigating hotair polymorphisms as a potential diagnostic markerfor type 2 diabetes in an iraqi population

Background: diabetes mellitus type 2 (t2dm) is a chronic condition characterized by insulin resistance, affecting approximately 530 million adults globally, with a prevalence of 10.5% among individuals aged 20 to 79. in iraq, the prevalence is significantly higher at 13.9%. objectives: this study examines the relationship between long noncoding rna hox transcript antisense rna (hotair) polymorphisms and t2dm risk, investigating hotair's potential as a diagnostic marker. methods: blood samples were collected from 28 t2dm patients and 20 healthy controls, with physiological parameters measured. hotairplasma levels were assessed using quantitative real-time (qrt)-pcr, and single-nucleotide polymorphisms rs12826786 and rs1899663 were analyzed through amplification refractory mutation system (arms)-pcr. results: hox transcript antisense rna expression was found to be 6.6 times higher in t2dm patients compared to controls, suggesting its involvement in t2dm pathophysiology. genotype distributions adhered to hardy-weinberg equilibrium, with the rs12826786 c allele appearing protective against t2dm, while rs1899663 showed no significant association. statistical analysis identified a significant relationship between the rs12826786 genotype and body mass index (bmi), though other diabetes-related metrics did not show significant results. conclusions: the findings suggest that elevated hotairexpression may play a role in t2dm, highlighting the need for further investigation into these associations and their potential implications for diagnosis and risk assessment.