anti-neoplastic effects of <i>juglans regia</i> l. green fruit pericarp on breast cancer cell lines

Background: recently, more attention has been paid to the use of complementary medicine in breast cancer (bc) treatment. juglansgreen fruit pericarp was found to have anti-cancer characteristics. objectives: we aimed here to assess the possible anti-proliferative effects of methanolic extract of juglansgreen fruit pericarp in bc cell lines. methods: mcf-7 and mda-mb-231 cells were examined with different concentrations of juglansgreen fruit pericarp extract. the cell viability was investigated using a 3 - 2, 5-diphenyl tetrazolium bromide (mtt) assay. also, different phases of the cell cycle were analyzed utilizing a flow cytometry method. finally, the ability of cancer cells to create colonies was studied post-treatment with juglansgreen fruit pericarp. results: our findings showed that the methanol extract has substantial cytotoxicity on mcf-7 and mda-mb-231 cell lines. we also observed a significant arrest in the g0/g1 phase in cancer cells post-treatment with the methanol extract. conclusions: in summary, our results introduced a cytotoxic property for juglansgreen fruit pericarp methanol extract for mcf-7 and mda-mb-231 cell lines. future studies are needed to further evaluate its utility as a potential complementary therapy for bc.