thymoquinone effects on the expression of p62 gene in cisplatin-induced testicular damage in mice

Background: cisplatin (cpn) is widely used for the management of various malignant tumors. objectives: this study investigated the effects of thymoquinone (tqn) on the expression of the p62 gene in cpn-induced testicular damage in mice. methods: histomorphometry, testis injury scores, expression of p62, and protein levels of lc3-ii were assessed. results: cisplatin induced histological changes, increased p62 expression (p < 0.01), and reduced lc3-ii levels (p < 0.001). thymoquinone pretreatment decreased p62 expression while increasing lc3-ii protein levels. thymoquinone significantly reversed the testicular injury scores and improved histomorphometric parameters. conclusions: the results indicate that tqn enhances autophagy and improves testicular tissue in cpn-intoxicated mice.