lncrna vldlr-as1 gene expression in colorectal cancer in patients from east azerbaijan province, iran

Background: colorectal cancer (crc) is the third most common cancer that frequently spreads to other parts of the body, with a low chance of recovery and a high mortality rate. long non-coding rnas are considered significant prognostic and diagnostic indicators because they are dysregulated in various cancers and have distinctive expression patterns and high tissue- and cell-specificity. vldlr-as1lncrna deregulation has been associated with several malignancies. objectives: this study aimed to evaluate the expression levels of vldlr-as1in crc. it was the first time this assessment was conducted. methods: we studied 188 samples, including 94 tumor samples and 94 paired adjacent non-tumor tissues. total rna was extracted, and its quantity and purity were assessed. takara primescript 1st strand cdna synthesis kit (kusatsu, japan) was used for the reaction. the steponeplus real-time pcr system (applied biosystems) was set up to assess the relative expression of vldlr-as1. results: according to the qrt-pcr data, the vldlr-as1expression levels in crc tissues were significantly lower than in tumor margins (p < 0.0001). in addition, receiver operating characteristic curve analysis demonstrated that vldlr-as1expression can discriminate between tumor and non-tumor samples with the sensitivity and specificity of 72.34% and 51.06%, respectively (p = 0.03, auc = 0.6274). however, no significant association was found between the expression levels of vldlr-as1and clinicopathological features in crc patients. conclusions: the results of this study indicated that vldlr-as1lncrna is significantly downregulated in the tumor tissues of crc patients compared to healthy tumor margin tissues. this evidence shows the potential of this gene as a promising biomarker for the early detection of crc development.