adipose-derived mesenchymal stem cells secretome induces apoptosis in colon carcinoma ht-29 cells

Background: recent studies have demonstrated that adipose mesenchymal stem cells (amscs)-derived secretome (amsc-se) has anticancer impacts. objectives: this study investigated the cytotoxic impacts of amsc-se on a colon carcinoma cell (ht-29) line. methods: the colon cancer cells were exposed to 50 or 100g/ml asmc-se for 24 hours. mtt test had used to examine the impacts of asmc-seonthe survival rates of the cells. caspase activity, mrna,and protein expression of bax and bcl-2 had evaluated to determine apoptosis. results: asmc-se could diminish the survival of the cells concentration-dependently. the mrna and protein expression of bax was concentration-dependently elevated, while bcl-2 expression decreased in the asmc-se group compared to the control concentration-dependently (p < 0.05). the caspase-3 and caspase-9 activities were concentration-dependently enhanced (p < 0.05), while the caspase-8 activity did not change with the amsc-se. conclusions: these findings indicate that amsc-se effectively prevents cell growth and induces apoptosis by stimulating the intrinsic apoptotic pathway in these cells.