high expression of twist-1 in the chronic myeloid leukemia patients with t315i mutation

Background: among the known abl mutations in chronic myeloid leukemia (cml), t315i is of particular importance. the t315i mutation may develop resistant cells that increase disease progression. twist-1 expression is impaired in patients with increased drug resistance. objectives: the current study aimed to measure the expression of twist-1 gene in cml patients to investigate its association with t315i mutation. methods: peripheral blood samples were taken from 40 cml patients. the expression of twist-1 and bcr-abl1 genes was quantified by real-time polymerase chain reaction (pcr). the gene expression was evaluated by rest software. cdna was used for amplification refractory mutation system (arms)-pcr reaction. results: of the 40 patients (age range: 19 - 72 years) participating in the study, 23 (57.7%) were female, and 17 (42.5%) were male. the expression of twist-1 gene was 43 ± 184.09-fold. the t315i mutation was detected in 3 (7.5%) patients. conclusions: according to our results, the twist-1 gene expression in patients with t315i mutation was significantly higher than patients without that mutation.