pulsed magnetic fields cause hes1 and hsa-circ-0068530 expression changes in gastric cancer cell line and human normal fibroblast cell line

Background: in recent years, the relationship between cancer cells and electromagnetic radiation has received much attention. objectives: the present study aimed to evaluate the effects of different intensities of electromagnetic fields on gastric cancer cell lines (ags). methods: after preparing ags and hu02 (normal) cell lines, they were exposed to magnetic flux densities of 0.25, 0.5, 1, and 2 millitesla (mt) for 18 h. the cell viability was studied by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay. the expression levels of hes1 and hsa-circ-0068530 rnas were studied by the quantitative real-time-pcr technique. results: the inhibition of gastric cancer cell line growth was observed under the influence of electromagnetic fields at different intensities. however, they did not affect the viability of normal cells. a sharp increase in the expression of hes1 and hsa-circ-0068530 genes was observed in normal cells exposed to 2 mt electromagnetic fields. conclusions: in general, it can be concluded that the effect of electromagnetic fields on gastric cancer cells depends on their intensity. magnetic flux densities of 0.25 and 0.5 mt had anti-cancer effects and magnetic flux density of 2 mt showed carcinogenic effects.