پاسخ بیوشیمیایی و فیزیولوژیکی گیاه کینوا به کاربرد سطوح مختلف نیتروژن و شوری آب آبیاری

به منظور بررسی اثرات کاربرد نیتروژن و آبیاری با زهاب مزارع نیشکر بر عملکرد دانه و ویژگی های فیزیولوژیک و بیوشیمیایی گیاه کینوا (رقم گیزاوان) شامل محتوای نسبی آب برگ، شاخص کلروفیل، فعالیت آنزیم های کاتالاز، سوپراکسیددیسموتاز، غلظت پرولین و عملکرد دانه آزمایشی مزرعه‌ای در سال زراعی 98-1397 به صورت کرتهای خرد شده در قالب طرح بلوک های کامل تصادفی در سه تکرار اجرا گردید. در این آزمایش چهار سطح کود نیتروژن (صفر، 75، 150، 225 کیلوگرم در هکتار) از منبع کود اوره به عنوان فاکتور اصلی و سه سطح آب آبیاری شامل شاهد (آب کارون با میانگین شوری 2.5 دسی زیمنس بر متر) و آبیاری یک در میان (کارون – زهاب نیشکر) و آبیاری با زهاب نیشکر (با میانگین شوری 7.5 دسی زیمنس بر متر) به عنوان فاکتور فرعی در نظر گرفته شد. نتایج تحقیق نشان داد شوری باعث کاهش معنی دار محتوای نسبی آب برگ، شاخص کلروفیل، عملکرد دانه و عملکرد بیولوژیکی و افزایش معنی دار فعالیت آنزیم های آنتی اکسیدان و نیز مقدار پرولین گردید. اما نیتروژن باعث افزایش معنی دار این صفات شد. تیمار 150 کیلوگرم نیتروژن در هکتار همراه با آبیاری یک در میان باعث حداکثر مقدار شاخص کلروفیل (44.81) و عملکرد دانه (2546 کیلوگرم در هکتار) گردید و بیشترین عملکرد بیولوژیکی گیاه (7468 کیلوگرم در هکتار) در تیمار 225 کیلوگرم نیتروژن در هکتار و آبیاری یک در میان مشاهده شد. بیشترین مقدار پرولین مربوط به تیمار 225 کیلوگرم کود اوره در هکتار با آبیاری زهاب (0.95 میلی‌گرم بر گرم وزن تر گیاهی) بود. نتایج این مطالعه تائیدی است بر این فرضیه، که با مصرف کود نیتروژن کافی می توان تا حدی از بروز اثرات زیان بار شوری بر گیاه کم نمود.

Biochemical and physiological response of quinoa to application of different levels of nitrogen and salinity irrigation water

IntroductionIn order to investigate the effects of nitrogen application and irrigation of sugarcane fields on grain yield and physiological and biochemical characteristics of quinoa (Gizavan cultivar) including relative leaf water content, chlorophyll index, catalase enzyme activity, superoxide dismutase, proline concentration and Field experimental grain yield in the cropping year 2018 was performed in the form of split plots in a randomized complete block design with three replications. The results showed that the maximum amount of chlorophyll index and grain yield were obtained in the application of 150 kg N ha1 with one irrigation with sugarcane. Application of 150 and 225 kg N ha1 under salinity stress caused a significant increase in the activity of catalase and superoxide dismutase enzymes.Materials and methodIn this experiment, four levels of nitrogen fertilizer (0, 75, 150, 225 kg ha1) from urea fertilizer source as the main factor and three levels of irrigation water including control (Karun water with salinity of 2.5 dS m1) and irrigation One in between (Karunsugarcane drainage) and irrigation with sugarcane drainage (with salinity of 7.5 dS m1) was considered as a subfactor.Seed sowing was done on November 6, 2018 in the form of barley and stack (on the grout line) and by hand. Each plot consisted of 6 planting lines 4 m long. The distance between the two plants was 710 cm and the distance between the lines was 50 cm. Irrigation treatments were applied in three to five leaf stage (seedling establishment stage). Before irrigation, soil moisture samples were taken and irrigation was done to reach the moisture content of the field capacity. For drainage irrigation, saline water of Mirza Kuchak Khan agroindustrial drains of Khorramshahr with salinity between 6 to 8 dS m1 was used. Plastic cover was used during rain. The final harvest was made in late February at the time of physiological maturation.At the flowering stage, samples of the leaves were prepared and immediately transferred to the laboratory for physiological and biochemical measurements.Results and discussionThe results of analysis of variance showed that the effects of nitrogen and salinity levels and their interaction at the level of one percent probability on the measured properties of quinoa were significant.The highest average relative leaf water in Karun irrigation and nitrogen fertilizer treatment was 225 kg ha1 (89.16%) and the lowest average of the mentioned trait was observed in drainage and nitrogen fertilizer treatment of 0 kg ha1 (70.76%).In the present study, salinity stress decreased the relative water content of the leaves by affecting water absorption because the relative water percentage of plant tissue is one of the most important components of plant water status. Quinoa has been shown to have the ability to accumulate salt ions in its tissues to control and regulate leaf water potential, and this reaction has enabled plants to maintain cell turbulence and limited transpiration under saline conditions, thus preventing physiological damage to quinoa under stress.due to the accumulation of more than 70% nitrogen in the chloroplast of plant leaves, the increase in nitrogen in the plant was accompanied by an increase in the concentration of chlorophyll and leaf nitrogen. Salinity stress due to irrigation with drainage and high accumulation of sodium in the plant has reduced the chlorophyll index and photosynthesis rate of quinoa and finally possibly the accumulation of oxygen free radicals in the plant has increased the activity of catalase enzyme with urea fertilizer catalase activity in all Salinity levels of irrigation treatments have increased due to the presence of nitrogen in the structure of the enzyme. The mechanism of the effect of salinity on the response of antioxidant enzymes is not yet fully understood.ConclusionsIn general, the results of this study indicate that changes in nitrogen levels more than irrigation levels had an effect on chlorophyll index, relative leaf water content and quinoa grain yield and the average salinity had a significant effect on increasing grain yield. Higher amounts of nitrogen increased but also led to a significant increase in grain yield. Nitrogen has improved the destructive effects of salinity stress in quinoa and increased enzyme activity in high salinity has increased plant tolerance. Due to high genetic diversity and adaptation to different climates, high nutritional value and high efficiency of resource use, quinoa can be a suitable plant for using unconventional water and soil resources of Khuzestan province.