تاثیر محرکهای نوری و اشعه Uv بر رشد رویشی و خصوصیات مورفولوژیکی گیاه Artemisia Aucheri Boiss در کشت آزمایشگاهی

عوامل اقلیمی بر رشد رویشی و مورفولوژیکی گیاهان تاثیرگذار هستند. نور از عوامل اقلیمی است و معمولاً بر خصوصیات ظاهری گیاهان تاثیر داشته که بر اساس میزان شدت نور و نوع گیاه متفاوت خواهد بود. هدف از این پژوهش بررسی تاثیر نور با فرکانس‌های متفاوت، بر رشد رویشی گیاه artemisia aucheri بود. جهت کشت از محیط کشت جامد موراشینگ و اسکوگ بدون تنظیم‌کننده‌های رشد استفاده شد. محیط کشت‌ها در شرایط استریل، تحت دمای 2±25 و شرایط نوری متفاوت با تیمارهای مختلف بودند. شرایط نوری استفاده از تابش‌های نوری 1000،2000 و 3000 لوکس بود. تیمار اشعه uv با شدت 320 نانومتر و تیمار تاریکی هم در نظر گرفته شد. برای تحلیل داده از نرم‌افزارهای spss نسخه 20 و برنامه excel استفاده شد. با توجه به آنالیز آماری نتایج، بین وزن تر و خشک کلیه نمونه‌ها رابطه مستقیمی وجود داشت. از طرفی بیشترین میزان وزن تر و خشک گیاهچه‌ها، در تیمار تحت تابش 3000 لوکس دیده شد. تاثیر این تیمار بر وزن گیاه با کلیه تیمارها و تیمار شاهد اختلاف معنی‌دار نشان داد و در تیمار تحت اشعه uv تغییرات ظاهری مختلفی دیده شد. نتایج نشان داد که بهترین شرایط نوری در این آزمایش تابش با شدت 3000 لوکس است که رشد طولی و رشد عرضی گیاه را بهبود بخشیده و حتی بر رشد ریشه‌ای تاثیر زیادی دارد. با افزایش شدت نور زمان رسیدن به حداکثر تراکم نیز کاهش یافت. از طرفی اشعه uv هم در شکل ظاهری گیاه و هم رشد ریشه‌ای و ساقه‌ای گیاه موثر بود.

Effect of light stimulators and UV on vegetative growth and morphological characteristics of the plant Artemisia aucheri Boiss. under laboratory culture

IntroductionThe traits and morphological characteristics of plants have a great impact on plant taxonomy and classification, so it is important to study these changes due to the impact of climatic factors. Climate factors can have impact on vegetative growth and the morphology of plants. One of the climatic factors is light, which will have a different effect on plant growth and morphology, based on the amount of light intensity and plant type. Investigating the effect of light with different intensities in a plant can help determine the proper light for that plant. Our goal in this paper was investigate the effect of light with different frequencies on growth of the plant Artemisia aucheri Boiss.Artemisia aucheri Boiss. Or Artemisia is a plant that is of family Asteraceae that has a large dispersal in northern Iran. This plant has different healing properties and thus it´s necessary examining and performing various experimental activities on the plant. As mentioned, the aim of this study was to investigate the changes in growth, against different light irradiation and UV radiation on this plant thus the effect of light with different frequencies on vegetative growth of Artemisia(Artemisia aucheri Boiss.), as well as the effect of UV rays with a wavelength of 320 nm, was investigated. Materials and methodsFor culture, Murashing and Skoog solid culture was used, without growth regulators. Culture medium was under sterile conditions, the temperature of 2 ±25 and different light conditions with different treatments. Lighting conditions was used of optical radiation 1000, 2000 and 3000 lux. In addition it was assumed radiation treatments UV and treatment of darkness. This experiment was repeated several times over a period of five months, with repeated repetitions, to prevent the possibility of a possible error. Each time the experiment was repeated, with three replicates of the treatments, and in addition, the apparent variation of plant changes, fresh and dry weight of the plants harvested, recorded and compared. For analyzing data of software SPSS version 20 and Excel programs were used. During the experiment, the resulting data, the average of three replicates ± SD and results based on oneway ANOVA test, Tukey test and a significant difference on the P ≤ 0.05, were investigated. Results and discussionThe results of the effect of light on fresh and dry weeds of seedlings showed that light radiation with different intensities caused a significant difference also UV rays can affect the fresh and dry weight of the plant. According to statistical analysis results between wet and dry weights of all samples, there was a direct relationship. However, the largest amount of fresh and dry weight of the seedlings was observed in treatment 3, the irradiation of 3000 lux treatments with all treatments and control treatments showed significant differences. Morphological changes were observed by UV treatment. Changes in the apparent symptoms of the plant due to the use of radiation can be expressed as deformation and color of leaves, leaf drying, cholorosis and necrosis in leaves (decreasing chlorophyll content), increasing leaf and stem thickness, decreasing stem elongation and root. The absence of light affects the outer shape of the plant, and its longitudinal growth rate. Plants that were in an environment without light usually had long, narrow stalks. However, the stems were yellow, but much longer and thinner than other treatments. The leaves did not spread much and were yellow. The plants that grew in the dark have a formidable appearance. Their shoots were longer and their diameter was smaller and pale. Increasing fresh and dry calluses in this treatment showed a significant difference with all treatments, and the weight gain was higher than the rest, except for 3000 lux. On the other hand, the colors of the calluses were creamy to the color of the coffee, and more were slim and sticky. Some parts of the callus were purple. ConclusionOne of the most important environmental conditions affecting plant growth and how it affects is light conditions. According to the information obtained, the intensity of light irradiation increases, the growth rate and plant density are improved and the plant weight is increased. The best lighting conditions in this experiment is 300 lux, the longitudinal growth and transverse growth of the plant, the better and even on root growth the impact a lot. Ray of UV the shape plant and root growth and stem of the plant was effective. With regard to the study of the irradiation of more than 3,000 lux in this experiment, it is suggested that higher radiation for this plant should be investigated in subsequent studies.