تغییرات فیزیولوژیکی، بیوشیمیایی و بیان ژن پروتئین کیناز وابسته به کلسیم در کلزا (.brassica napus l) تحت تنش شوری

تنش شوری یکی از مهمترین تنش‌های غیرزیستی می‌باشد که عملکرد کلزا را تحت تاثیر قرار میدهد. به منظور مطالعه برخی از پاسخ های فیزیولوژیکی کلزا و تغییرات بیان ژن bnacdpk14 در شرایط تنش شوری، دو رقم متحمل (slm046 و زرفام) و دو رقم حساس (اکاپی و ساریگل) در اتاقک رشد کشت شدند و تنش شوری از طریق آبیاری با آب حاوی 100 و 200 میلی‌مولار نمک (nacl) اعمال شد. محتوای نسبی آب برگ، نشت یونی، آنزیم گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن پروتئین کیناز وابسته به کلسیم 14 کلزا اندازه‌گیری شدند. در شرایط شوری بالاتر (200 میلی مولار نمک کلرید سدیم) محتوای نسبی آب کاهش و نشت یونی افزایش یافت. فعالیت آنزیم گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن bnacdpk14 نیز تحت تنش افزایش یافت که مقدار این افزایش در ارقام متحمل بیشتر از ارقام حساس بود. همبستگی بالای منفی بین صفات محتوای نسبی آب برگ و نشت یونی مشاهده شد. همبستگی بالا مثبت بین صفات گایاکول پراکسیداز، آنزیم کاتالاز و بیان ژن bnacdpk14 وجود داشت که نشان می دهد با افزایش گونه‌های فعال اکسیژن، گیاه برای مقابله با آن سیستم آنتی اکسیدان خود را بکار می‌گیرد.

Physiological and biochemical changes and calcium-dependent protein kinase expression in canola (Brassica napus L.) under salinity stress

Salinity stress is one of the most important of abiotic stress that affects the yield of oilseed rape. In order to study some physiological and biochemical changes and BnaCDPK14 transcript expression in rapeseed (Brassica napus L.), two tolerant cultivars (Slm046 and Zarfam) and two susceptible cultivars (Okapi and Sarigol) were planted in a growth chamber and were irrigated by water including 100 and 200 mM NaCl and normal water. Relative water content, electrolyte leakage, antioxidant enzyme guaiacol peroxidase (GPOX), antioxidant enzyme catalase (CAT) and the expression of calciumdependent protein kinase 14 (BnaCDPK14) were measured. The results indicated the relative water content and electrolyte leakage (200 mM NaCl) decreased and increased under stress respectively. The antioxidant enzyme guaiacol peroxidase (GPOX), catalase (CAT) and BnaCDPK14 increased by salinity stress, tolerant cultivars showing more increase. Negative correlation was observed between the relative water content of leaves and electrolyte leakage. There was a high positive correlation between the guaiacol peroxidase and catalase contents and the expression of BnaCDPK14, indicating that by increasing the reactive oxygen species under stress, the plant enzymic antioxidant system helps the plant to cope with it. Salinity stress is one of the most important of abiotic stress that affects the yield of oilseed rape. In order to study some physiological and biochemical changes and BnaCDPK14 transcript expression in rapeseed (Brassica napus L.), two tolerant cultivars (Slm046 and Zarfam) and two susceptible cultivars (Okapi and Sarigol) were planted in a growth chamber and were irrigated by water including 100 and 200 mM NaCl and normal water. Relative water content, electrolyte leakage, antioxidant enzyme guaiacol peroxidase (GPOX), antioxidant enzyme catalase (CAT) and the expression of calciumdependent protein kinase 14 (BnaCDPK14) were measured. The results indicated the relative water content and electrolyte leakage (200 mM NaCl) decreased and increased under stress respectively. The antioxidant enzyme guaiacol peroxidase (GPOX), catalase (CAT) and BnaCDPK14 increased by salinity stress, tolerant cultivars showing more increase. Negative correlation was observed between the relative water content of leaves and electrolyte leakage. There was a high positive correlation between the guaiacol peroxidase and catalase contents and the expression of BnaCDPK14, indicating that by increasing the reactive oxygen species under stress, the plant enzymic antioxidant system helps the plant to cope with it. Salinity stress is one of the most important of abiotic stress that affects the yield of oilseed rape. In order to study some physiological and biochemical changes and BnaCDPK14 transcript expression in rapeseed (Brassica napus L.), two tolerant cultivars (Slm046 and Zarfam) and two susceptible cultivars (Okapi and Sarigol) were planted in a growth chamber and were irrigated by water including 100 and 200 mM NaCl and normal water. Relative water content, electrolyte leakage, antioxidant enzyme guaiacol peroxidase (GPOX), antioxidant enzyme catalase (CAT) and the expression of calciumdependent protein kinase 14 (BnaCDPK14) were measured. The results indicated the relative water content and electrolyte leakage (200 mM NaCl) decreased and increased under stress respectively. The antioxidant enzyme guaiacol peroxidase (GPOX), catalase (CAT) and BnaCDPK14 increased by salinity stress, tolerant cultivars showing more increase. Negative correlation was observed between the relative water content of leaves and electrolyte leakage. There was a high positive correlation between the guaiacol peroxidase and catalase contents and the expression of BnaCDPK14, indicating that by increasing the reactive oxygen species under stress, the plant enzymic antioxidant system helps the plant to cope with it.