Effect of Some Synthetic Parameters on Size and Polydispersity Index of Gelatin Nanoparticles Cross-Linked by CDI/NHS System

In  our previous work,  the  effect  of use of  a water  soluble cdi/nhs system  as  nontoxic  cross-linking  agent  on  fabrication  of  gelatin nanoparticles was investigated. in this research, the effect of variation in some synthetic parameters of gelatin nanoparticles cross-linked by cdi/nhs  system  such  as  type  of  gelatin  and  formulation  of  cross-linking  agent  on  their  size  and  distribution  was  examined.  the conventional two step desolvation method was used for preparation of gelatin  nanoparticles.  the  morphology,  mean  size  and  size distribution of the formed nanoparticles were evaluated and compared with  each  other.  in  addition,  intrinsic  viscosities  of  all  the nanoparticles  were  measured  and  compared  under  different conditions.  the  results  showed  that  the  presence  of more nhs  and absence of nhs catalyst in cdi/nhs system lead to the large particle size and broad size distribution of nanoparticles that were attributed to the fast and slow cross-linking rate, respectively.